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Figure 3 | BMC Cancer

Figure 3

From: BIMELis a key effector molecule in oxidative stress-mediated apoptosis in acute myeloid leukemia cells when combined with arsenic trioxide and buthionine sulfoximine

Figure 3

BSO induces conformational change in BAX, but not BAK. The conformational change was detected using immunoprecipitation and immunoblotting with an antibody to conformationally changed BAX or BAK. HL60 cells were treated with ATO/BSO or ATO for 12 h in the presence or absence of DTT. Actin and IgG light chain (IgG(L)) were used as the controls. A typical result of 3 independent experiments is shown. IP, immunoprecipitation; Ppt, immunoprecipitate; Sup, supernatant.

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BMC Cancer

ISSN: 1471-2407

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