Figure 3
Determination of extracellular levels of TG2 in response to resveratrol. Representative immunofluorescence images showing extracellular TG2 level (red) in SHYvector, SHYTG2, and SHYmutant (A), and Panc-28 and Hs766T cells (B). After 48 h with or without resveratrol (1 or 10 μM) treatment in scratch assays, extracellular TG2 was detected using anti-TG2 antibody. Nuclei were stained with DAPI and visualized under fluorescence microscopy. Pictures are representative of at least 3 independent experiments. Scale bar = 100 μm.