Table 1 Antibodies and conditions used for immunohistochemistry assays
From: Evaluation of protein biomarkers of prostate cancer aggressiveness
Antibody | Specificity | Clone | Dilution | Antigen retrieval | Incubation | Detection | Source | Cat # |
|---|---|---|---|---|---|---|---|---|
ACPP ACPP | Polyclonal (rabbit) | - | 1:2500 | Steamer/Citrate pHÂ 6 | 1Â h | Novocastra Novolink Polymer | Sigma-Aldrich | HPA004335 |
ADAM9 | Polyclonal (rabbit) | - | 1:100 | Steamer/Citrate pHÂ 6 | 1Â h | Novocastra Novolink Polymer | Sigma-Aldrich | HPA004000 |
ALDH1A2 | Polyclonal (rabbit) | - | 1:1000 | Steamer/Citrate pHÂ 6 | 1Â h | Novocastra Novolink Polymer | Sigma-Aldrich | HPA010022 |
CASR | Polyclonal (rabbit) | - | 1:200 | Steamer/Citrate pHÂ 6 | 1Â h | Novocastra Novolink Polymer | Abcam | ab18200 |
CCND1 | Monoclonal (mouse) | DCS-6 | 1:50 | Decloaker/Citrate pHÂ 6 | 1Â h | Novocastra Novolink Polymer | Dako | M7155 |
CCPG1 | Polyclonal (rabbit) | - | 1:400 | Steamer/Citrate pHÂ 6 | 1Â h | Novocastra Novolink Polymer | Sigma-Aldrich | HPA026861 |
CD34 | Monoclonal (mouse) | QBEnd/10 | 1:4000 | Steamer/Citrate pHÂ 6 | 1Â h | Novocastra Novolink Polymer | Neomarkers | MS-363-P |
CD44 | Monoclonal (mouse) | SP37 | 1:400 | Steamer/Citrate pHÂ 6 | O/N | Novocastra Novolink Polymer | Dako | M708201-2 |
CD44v6 | Monoclonal (mouse) | 2Â F10 | 1:1000 | Steamer/Citrate pHÂ 6 | 1Â h | Novocastra Novolink Polymer | R&D Systems | BBA13 |
CHGA | Monoclonal (mouse) | LK2H10 + PHE5 | 1:800 | Steamer/Citrate pH 6 | 1 h | Novocastra Novolink Polymer | Biocare Medical | CM010B |
CHMP1A | Polyclonal (rabbit) | - | 1:100 | Steamer/Citrate pHÂ 6 | 1Â h | Novocastra Novolink Polymer | Sigma-Aldrich | HPA006776 |
EI24 | Polyclonal (rabbit) | - | 1:8000 | Steamer/Citrate pHÂ 6 | 1Â h | Novocastra Novolink Polymer | Sigma-Aldrich | SAB1100756 |
ENO2 | Monoclonal (mouse) | 5E2 | 1:800 | No AR | 1Â h | Novocastra Novolink Polymer | Leica | NCL-L-NSE2 |
GADD45B | Polyclonal (rabbit) | - | 1:25 | Steamer/Citrate pHÂ 6 | 1Â h | Novocastra Novolink Polymer | Sigma-Aldrich | HPA029816 |
HABPa | NA (bovine) | - | 1:400 | No AR | O/N | VECTASTAIN Elite ABCb | Calbiochem | 385911 |
HAS2 | Polyclonal (goat) | - | 1:1000 | Steamer/Citrate pHÂ 6 | 1Â h | VECTASTAIN Elite ABC | Santa Cruz | sc-34067 |
HES6 | Polyclonal (rabbit) | - | 1:1000 | Steamer/Citrate pHÂ 6 | O/N | Novocastra Novolink Polymer | Abcam | ab66461 |
HMMR | Monoclonal (mouse) | 6B7D8 | 1:900 | Steamer/Citrate pHÂ 6 | 1Â h | Covance Ultra Streptavidin-HRP | ProMab | NA |
HOXC6 | Polyclonal (rabbit) | - | 1:200 | Steamer/Citrate pHÂ 6 | 1Â h | Novocastra Novolink Polymer | Santa Cruz | sc-66925 |
HYAL1 | Polyclonal (rabbit) | - | 1:100 | Steamer/Citrate pHÂ 6 | O/N | Novocastra Novolink Polymer | Sigma-Aldrich | HPA002112 |
IGF1 | Polyclonal (rabbit) | - | 1:400 | Steamer/Citrate pHÂ 6 | 1Â h | Novocastra Novolink Polymer | Millipore | 07-1411 |
IQCK | Polyclonal (rabbit) | - | 1:70 | Steamer/Citrate pHÂ 6 | 1Â h | Novocastra Novolink Polymer | Sigma-Aldrich | HPA026792 |
MAP4K4 | Polyclonal (rabbit) | - | 1:100 | Steamer/Citrate pHÂ 6 | 1Â h | Novocastra Novolink Polymer | Sigma-Aldrich | HPA008476 |
MKI67 | Monoclonal (mouse) | MM1 | Predilute | Decloaker/CCS1 | 32Â min | Ventana ultraView Universal | Leica | ORG-8772 |
PAGE4 | Polyclonal (rabbit) | - | 1:2000 | Steamer/Citrate pHÂ 6 | 1Â h | Novocastra Novolink Polymer | Sigma-Aldrich | HPA023880 |
PLIN2 | Polyclonal (rabbit) | - | 1:100 | Steamer/Citrate pHÂ 6 | 1Â h | Novocastra Novolink Polymer | Abbiotec | 251533 |
PTEN | Polyclonal (rabbit) | - | 1:200 | Steamer/Citrate pHÂ 6 | 1Â h | Novocastra Novolink Polymer | Invitrogen | 18-0256 |
SIAH2 | Monoclonal (mouse) | 24E6H3 | 1:50 | Steamer/Citrate pHÂ 6 | 1Â h | Novocastra Novolink Polymer | Novus | NB110-88113 |
SMAD4 | Monoclonal (rabbit) | EP618Y | 1:100 | Steamer/Citrate pHÂ 6 | 1Â h | Novocastra Novolink Polymer | Millipore | 04-1033 |
SOX9 | Polyclonal (rabbit) | - | 1:200 | Steamer/Citrate pHÂ 6 | 1Â h | Novocastra Novolink Polymer | Sigma-Aldrich | HPA001758 |
SPP1 | Monoclonal (rabbit) | EPR3688 | 1:50 | Steamer/Citrate pHÂ 6 | 1Â h | Novocastra Novolink Polymer | Epitomics | 2671-1 |
SYP | Monoclonal (mouse) | 27G12 | 1:400 | Steamer/Citrate pHÂ 6 | 1Â h | Novocastra Novolink Polymer | Leica | NCL-SYNAP-299 |
TP53 | Monoclonal (mouse) | DO-7 | 1:40000 | Steamer/Citrate pHÂ 6 | 1Â h | Novocastra Novolink Polymer | Dako | M7001 |
| Â | Monoclonal (mouse) | Bp53-11 | Predilute | Decloaker/CCS1 | 16Â min | Ventana ultraView Universal | Ventana | 760-2542 |