Figure 6

Tpr-Met-mediated enhanced growth in IECs is reduced by inactivation of MEK1/2, and resistance to anoikis is restored by dual inactivation of MEK1/2 and PI3K. (A) Cell-count assays were performed with Tpr-Met and Control-IEC-6 cells cultured under adherent conditions and in presence of serum, after 24 and 48 hours of treatment with vehicle (DMSO), 10 μM of the MEK1/2 or PI3K inhibitor, or a combination of both inhibitors. The bar graph shows as percentage the mean number of cells ± S.E.M. relative to DMSO-treated cells. (B) Anoikis assays were conducted 24 and 48 hours after seeding Tpr-Met-IEC-6 cells in suspension, and concurrently in the presence of the vehicle or indicated inhibitors. The bar graph represents in percentage the mean value ± S.E.M. of cell viability normalized to DMSO-treated cells, calculated from 3 independent experiments performed in triplicate.