Figure 13

Ceramide analog suppresses breast cancer growth and spontaneous lung metastasis. A. LCL85 suppresses breast cancer growth and metastasis. 4 T1 cells were injected to the mammary fat pad of mice. Tumor bearing mice were treated with LCL85 (2.5 mg/kg body weight) through i.v. injection. Tumor sizes were recorded and presented at the bottom left panel. The tumors were also dissected and weighed and presented at the bottom right panel. Column, mean; bar, SD. B. Lungs of tumor-bearing mice as in A were analyzed for tumor nodules. The white spots are tumor nodules and the black tissues are normal lung tissues. Shown are images of representative tumor-bearing lungs. The tumor nodules in each lung were enumerated and presented at the right panel. Column, mean; bar, SD. C. LCL85 suppresses spontaneous breast cancer metastasis. 4 T1 cells were transplanted to the mammary fat pads of mice. Primary tumors were surgically removed 15 days later. Mice were treated with LCL85 (2.5 mg/kg body weight) at days 8, 11, 14 and 17 after surgery. Lungs were analyzed for metastasis at day 19 after surgery. Shown are tumor-bearing lungs. D. The tumor nodules were enumerated. Each dot represents total tumor nodule number of a mouse lung.