Figure 12

LCL85 suppresses colon carcinoma cell metastatic potential in vivo . A. Colon26 cells were injected i.v. into mice. Tumor-bearing mice were treated with LCL85 at the indicated doses every 3 days for a total of 4 times. Mice were sacrificed and examined for lung metastases nodules. Shown are tumor-bearing lungs from one of two experiments. B. The number of lung tumor nodules are enumerated and shown. Each dot represents total counts from a single mouse. Counts greater than 250 are expressed as >/= 250. The horizontal lines in the plot box represent mean tumor nodule numbers. C. Analysis of FasL⁺ cells in tumor-bearing lungs. Single cell suspensions were prepared from lungs of tumor-bearing mice as shown in A, stained with CD8- and FasL-specific mAbs and analyzed by flow cytometry. IgG isotype was used as a negative control (left panel). CD8+ T cells (middle panel), FasL + and CD8+ T cells (right panel) are indicated in each plot. Shown are representative results of 1 from 3 mice. D. Analysis of FasL⁺ cells in lungs of tumor-free mice. Single cell suspensions were prepared from lungs of tumor-free mice, stained with CD8- and FasL-specific mAbs and analyzed by flow cytometry. IgG isotype was used as a negative control (left panel). FasL + and CD8+ T cells (right panel) are indicated in each plot. Shown are representative results from 1 of 4 mice.