Cell surface knockdown of MUC16 in OVCAR3 sublines expressing ER-targeted anti-MUC16 scFvs. Ctrl scFv-expressing cell line and MUC16 knockdown (1:9#7 scFv and 1:9#9 scFv) cell were assessed for MUC16 expression by immufluorescence with anti-CA125 M11 antibody. (A) The expression of MUC16 in non-permeabilized cells was determined by immunofluorescence. Cells were incubated with the anti-CA125 M11 antibody and nuclei were stained with DAPI. (B) Immunoblot analysis of MUC16 expression. Equal loading was confirmed using an anti-tubulin antibody.