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Table 2 Degree of inter-operator agreement in scoring of CTC for expression of the androgen receptor by immunohistochemistry

From: Optimisation of an immunohistochemistry method for the determination of androgen receptor expression levels in circulating tumour cells

  1stRun 2ndRun
Evaluation Kappa1 % Agreement Kappa % Agreement
Positive v Negative2 0.94 97.2 0.98 98.9
Staining intensity3 0.59 75.0 0.81 87.9
  1. 1Kappa values where calculated as described in Methods where the agreement between operators is defined as follows: < 0 none, 0–0.20 slight, 0.21–0.40 fair, 0.41–0.60 moderate, 0.61–0.80 substantial, and 0.81–1 almost perfect [22].
  2. 2This table entry refers to an overall assessment of the level of positive versus negative staining regardless of the staining intensity.
  3. 3Staining intensity was graded into 4 categories as negative, weak (1 +), moderate (2 ++) or strong (3 +++).