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Figure 3 | BMC Cancer

Figure 3

From: Optimisation of an immunohistochemistry method for the determination of androgen receptor expression levels in circulating tumour cells

Figure 3

Typical example of the training set of images used to standardise AR receptor staining intensity in CTC isolated by ISET. After identifying significant operator bias in assigning staining intensities (see Figure 2), a standard gallery of 20 different images was produced as an aid to staff training. The figure contains a typical set of images from the gallery each containing a single patient derived CTC isolated on an ISET membrane and stained for AR expression by IHC to illustrate the different levels of staining intensity observed and the scoring system utilised in the present study.

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