Figure 7

Oridonin represses the growth of NOZ cells in athymic nude mice. NOZ cells were subcutaneously injected into the right flank of the nude mice. The mice were then administered 0.2 mL of vehicle (10% DMSO and 90% PBS) or oridonin (5, 10, and 15 mg/kg) intraperitoneally every 2 days for up to 3 weeks. (A) Photos of 5 representative mice (n = 10) from each group were presented to show the sizes of the resulting tumors. (B) Tumor dimensions were periodically measured using calipers. (C and D) Tumors were excised from the animals and weighed. The data are expressed as the mean ± SD (n = 10). *P < 0.05, **P < 0.01 vs. the control group. (E) The expression of apoptosis-related proteins in tumor tissue was also assessed by western blot analysis, and β-actin was used as a loading control. The results shown were representative data from 3 independent experiments.