Figure 4From: Oridonin induces apoptosis and cell cycle arrest of gallbladder cancer cells via the mitochondrial pathway Oridonin induces apoptosis in gallbladder cancer cells. SGC996 and NOZ cells were treated with oridonin (7.5, 15, and 30 μmol/L) for 48 h. (A) Oridonin-treated SGC996 and NOZ cells were stained with annexin V-FITC/PI and analyzed by flow cytometry. (B) The percentage of apoptotic cells is presented as the mean ± SD (n = 3). (C) Changes in apoptotic nuclear morphology were observed by Hoechst 33342 staining and visualized by fluorescent microscopy. Results shown were representative data from 3 independent experiments. *P < 0.05, **P < 0.01 vs. the control group.Back to article page