Evaluation of time- & ER-dependency in predicting Distant Metastasis Free Survival (DMFS) by gene signatures (n = 912). Estimated effect (standardized hazard ratios, eβ, with 95% confidence intervals) of gene signatures for survival prediction within different time intervals and stratified by ER status. The X-axis indicates the follow-up time intervals: up to 5-year, 5–10 year, and beyond 10 year. Within each subinterval, a univariate Cox model per signature was fitted. The Y-axis indicates the estimated hazard ratios (HR) on a logarithmic scale corresponding to a 1 standard deviation increase in the signature. The null, HR = 1, is indicated by the blue line. Solid dots indicate HRs significantly different from 1 (P < 0 · 05). ER + (n = 692) is denoted as red and ER– (n = 220) is denoted as blue. The number of events for each follow-up subinterval in ER + subgroup is 126, 41 and 10, respectively; and in ER- subgroup 61, 7 and 5, respectively.