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Figure 4 | BMC Cancer

Figure 4

From: Increased diacylglycerol kinase ζ expression in human metastatic colon cancer cells augments Rho GTPase activity and contributes to enhanced invasion

Figure 4

Stable shRNA expression reduces DGK ζ levels in SW620 cells. (A) Equivalent amounts of protein from detergent extracts of SW620 cells, SW620 cells stably expressing an empty lentiviral vector (Vector1 and 2), or SW620 cells expressing an shRNA against DGKζ (shRNA1 and 2) were immunoblotted with anti-DGKζ (top) and anti-tubulin (bottom) antibodies. (B) Graph showing DGKζ protein levels in each cell line as measured by densitometric analysis of western blots. The data were normalized to the level of tubulin and are expressed as a percentage of DGKζ in SW620 cells. Values are the average of at least three independent experiments ± S.E.M. Statistical analysis was performed by a one-way ANOVA followed by a Tukey post-hoc multiple comparison test. The asterisks denote a highly significant difference (P < 0.001) from SW620 cells.

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