Figure 6

Expression of AKT1m in normal breast tissues and selected breast cancer specimens. qRT-PCR analysis to determine the relative expression of the AKT1m transcript variants in three primary human breast tissues and nine breast cancers from different patients, including three ERα-positive as well as three Her2-positive/ERα-negative and three triple-negative specimens. Additional samples from other organs (i.e., lung, liver, pancreas, and stomach) were included into this analysis to assess a possible widespread or tissue-specific expression of the AKT1m transcripts. Note there was a clear elevation in the expression of AKT1m in the lung and stomach of two patients who were diagnosed with pneumonia and a GI stromal tumor, respectively (circles). Each sample was examined in triplicate in the qPCR assay and normalized to GAPDH expression; bars represent SEM.