Figure 5

Immunohistochemical studies. A) Very low KIAA1199 immunostaining (first row) in MDA-MB-231-ShB tumor comparing to the controls (×4). Representative illustration of immunohistochemical studies (×100 magnifications) shows the higher expression level of KIAA1199 (brown staining cells in the second row), lower apoptosis activity (CASP3, third row) and higher proliferation activity (PCNA, fourth row) than the MDA-MB-231-ShB tumor. B) Evaluation of the expression of protein markers by calculation of immunostaining index using the Metamorph software; graphs from left to right show the relative expression of KIAA1199, CASP3 and PCNA in control versus KIAA1199 knockdown tumor sections.