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Table 1 VM signaling-related markers

From: Norcantharidin inhibits tumor growth and vasculogenic mimicry of human gallbladder carcinomas by suppression of the PI3-K/MMPs/Ln-5γ2 signaling pathway

Gene PCR primers (forward-reverse) Amplification size (bp) Cycle no.
PI3-K 5′-TGTCGCAGCCCAGGTAGATT-3 269 35
5′-CAGGAGGTGGTCGGGTCAAG3-′
MMP-2 5′-TCTGAGGGTTGGTGGGATTGG-3 290 35
5′-AAGAGCGTGAAGTTTGGAAGCA-3′
MM1-MMP 5′-CAAAGGCAGAACAGCCAGAGG3-′ 180 35
5′-ACAGGGACCAACAGGAGCAAG-3′
Ln-5γ2 5′-ACACGGGAGATTGCTACTCG3-′ 123 35
5′-ACCCATTGTGACAGGGACAT-3′
GAPDH 5′-CCTCTATGCCAACACAGTGC-3′ 211 35 ~ 40
5′GTACTCCTGCTTGCTGATCC-3′