Expression of MMP-2, MT1-MMP proteins from sections [(A), SABC method, original magnification, ×200)] and supernates [(B), ELISA] of the 3-D cultures of GBC-SD cells
. (A) The positive expression site of MMP-2 and MT1-MMP proteins presented yellow-brown reactant was in cytoplast. Expression of MMP-2 and MT1-MMP proteins in TIMP-2 group and NCTD group was significantly lower than that of control group (all *P < 0.001). (B) Expression of MMP-2 and MT1-MMP proteins from supernates of 3-D culture samples in control group increased significantly as time prolonged, when compared with TIMP-2 group or NCTD group (all *P < 0.001).