Figure 3

MET and RON are localized to the same cellular compartment that is evolutionarily conserved. A. The colocalization of phospho-MET (active form of MET) with PAX8 transcription factor in the peri-nuclear area with HGF stimulation. Representative confocal pictures showing individual staining patterns for phospho–MET and PAX8 and the two merged in control (left panel) and HGF treated (right panel) in A549 cells are shown. The cells were treated with HGF for 15 min at concentration of 100 ng/ml. B. The colocalization of phospho-RON (active form of RON) with PAX8 transcription factor in the peri-nuclear area with HGF stimulation: Representative confocal pictures revealing staining patterns of p-RON and PAX8 as well as their merged pictures are shown. C: Colocalization of PAX8 with MET and RON in C. elegan’s embryos. Immunofluorescence of C. elegans was performed as described in Methods. The left panel shows representative confocal pictures of C. elegan’s embryos stained with DAPI, PAX8, MET and merged. The right side panel shows staining with DAPI, PAX8, RON, and merged.