Figure 2

Effect of PAX8 knockdown on the expression of PAX8, PAX2, MET and RON in A549 cells. A549 cells were transfected with scrambled and PAX8 specific siRNA at 100nM concentration. The whole cell lysates were prepared and then immunoblotted with anti-PAX8, anti-PAX2 antibodies. The lysates were used in parallel to determine the MET, RON, and EGFR expressions using specific antibodies in immunoblotting. β-actin was used as a loading control. As shown, the knockdown of PAX8 had no effect on PAX2 levels. However a precipitous decrease in both RON and MET expression levels was observed.