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Figure 1 | BMC Cancer

Figure 1

From: Dissecting the signaling pathways associated with the oncogenic activity of MLK3 P252H mutation

Figure 1

Expression profiling of biological triplicates of HEK293 cells transfected with mutant and wild-type MLK3. (a) Heatmap of the 445 genes which clearly classify the mutant vs the wild-type, selected on the basis of a 2 log-fold change in expression between cells transfected with P252H mutant and wild-type forms of MLK3, and eliminating genes which were differentially expressed between the Mock and wild-type transfection (red=down-regulated in P252H mutant MLK3, green=up-regulated in P252H mutant MLK3). (b) Illustrates a subset of the heatmap, focusing on genes which are part of the colorectal cancer pathway which is significantly affected (p-value = 0.03). (c) Graphical representation of the colorectal pathway (larger rounded rectangle) and relevant pathways contained within it (smaller rounded rectangles), such as MAPK, WNT, TGF-β, NOTCH and p53, indicating differentially expressed genes (ovals) present in Figure 1, their relationships to each other (solid line indicates a direct protein-protein interaction, T shaped ending for inhibition interactions, arrow ending for activation interactions, and no ending for other known interactions), as well as the direction of the expression change (red = down-regulated in P252H mutant MLK3 transfection, green = up-regulated in mutant MLK3). White Diamond shaped boxes indicate entire gene families, which are significantly affected, genes within the families are shown as colored ovals. Long dashed lines indicate genes, which are present in multiple pathways. (d) Graphical representation of Real-time PCR quantification of mutant vs wild-type MLK3 targets (LEF1, CCND1, BMP6 and FZD10) selected from expression microarrays.

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