Figure 4

Response to IR in HEC-6 PTEN + and parental HEC-6 cell lines. (A) Total/cleaved PARP and total/phospho-PTEN expression in HEC-6 PTEN + and parental HEC-6 cell lines were examined by western blot analysis. Proteins were extracted after 10 Gy of IR at the indicated times. (B) Immunofluorescence images of PTEN-/+ HEC-6 cell lines: Hoechst-stained nuclei (blue), γ-H2AX (red), and RAD51 foci (green) after IR exposure (2 Gy). (C) The number of γ-H2AX and RAD51 foci following exposure to IR (2 Gy) was counted in the PTEN-/+ HEC-6 cell lines. The experiments were repeated 3 times, and each value is shown as the mean of 3 experiments ± SD. (D) Clonogenic assay in the PTEN-/+ HEC-6 cell lines after exposure to IR at the indicated doses (2–6 Gy).