Figure 3
Cell proliferation assay. Cells were cultured in quadruplicate in a 96-well plate at a density of 1000 cells/per well, and proliferation was measured by Cell Titter-Glo® cell viability assay. Growth curve of CD24+/CD44+ and CD24-/CD44+ subpopulations of A253 cells (A) and KCCT873 cells (B) are shown. Data represent mean ± SD of triplicate determinations. P value is shown for day 5 time point.