Figure 2

Differentiation of CD24+/CD44+ cells. (A) A253 CD24+ HNSCC cells differentiate into CD24-cells. Population dynamics modeled by a simple growth model in which CD24+ cells divide and switch to a CD24-state. Flow cytometry plots illustrate the sorted CD24+ cell populations at week one, two and three, from left to right panels. (B) Flow sorted CD24+ cells were monitored for 3 weeks in cell culture for their ability to convert into CD24-cells. Day 0 indicates the day cells were sorted by CD24 expression. The percentage of the CD24+ cells decreased in a time-dependent manner.