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Figure 1 | BMC Cancer

Figure 1

From: Identification and characterization of cancer stem cells in human head and neck squamous cell carcinoma

Figure 1

Expression of CD24 and CD44 in A253 HNSCC cells. (A) Flow cytometric analysis of CD24+ and CD44+ cells in A253 HNSCC cell line. Dual staining of A253 HNSCC cells indicate that CD24+/CD44+ subpopulation is ~6%, while CD24-/CD44+ subpopulation is >93% in the whole cell population. qRT-PCR analysis of stemness-related genes in FACS-sorted CD24+/CD44+ and CD24-/CD44+ cells derived from A253 (B) and KCCT873 (C) tumor cells. Data represent log2 mean fold changes in gene expression ± SD of triplicate determinations in CD24+/CD44+ compared to CD24-/CD44+ subpopulations from both cell lines. P values for two genes, BMI1 and Nanog, in two cell lines are shown.

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