Skip to main content
Figure 2 | BMC Cancer

Figure 2

From: Metformin enhances tamoxifen-mediated tumor growth inhibition in ER-positive breast carcinoma

Figure 2

Metformin and tamoxifen inhibit the DNA replication activity of MCF-7 and ZR-75-1 cells. (A and C) MCF-7 and ZR-75-1 cells were treated with 5 or 10 μM Tam, 5 mM Met, or 5 or 10 μM Tam + 5 mM Met. After 2 days, the DNA replication activity of the cells was measured by BrdU assay. A red color indicates cells in the DNA replication phase, and a blue color indicates the cell nucleus (scale bar = 20.0 μm). (B and D) BrdU-positive cells were counted in five random high-power fields (original magnification, 400x). Histograms represent quantification of BrdU-positive cells. All data are expressed as the mean ± standard deviation (SD) for three independent experiments. *P < 0.05 for the 5 μM Tam group vs. the 5 μM Tam + 5 mM Met group; # P < 0.05 for the 10 μM Tam group vs. the 10 μM Tam + 5 mM Met group.

Back to article page