Figure 3

Platelet depletion increases vessel permeability. (A) Orthotopic implantation of B16/F10 tumor cells into C57B6/L mice were followed by injections every 3 days of GPI or control antibody after the tumors reached ~500 mm³. Evans blue was then injected (100 μL 5% Evans blue, i.v.; 30 min) at the experimental endpoint (24 days). Evans blue was then extracted and quantified spectrophotometrically at 620 nm. *,p < 0.05. (B) Photographs of Evan’s blue dye leakage 30 minutes following intradermal injection of either saline or VEGF into the shaved dorsal skin of control and platelet depletion mice. n = 3 for each group. (C) Comparison of intratumoral hemoglobin content among control and PLT-depleted mice, *p < 0.05. (n = 6 for each group). (D) Western blot analysis of VE-cadherin expression in tumors from control and PLT-depleted mice. Quantification of Western blot analysis for VE-cadherin (normalized to β-actin).