Figure 5

Cellular changes in mPEPCK-suppressed SNU-C4 cells. (a) No change in the mitochondrial membrane potential or apoptotic cell death was observed in SNU-C4 transfected with mPEPCK siRNA. Flow cytometry was performed using JC-1 to determine the mitochondrial membrane potential and apoptotic cell death rate. (b) No changes in apoptosis-related mitochondrial proteins were observed in the non-transfected, NS, or mPEPCK siRNA-transfected SNU-C4 cells. Whole cellular homogenate, mitochondrial, and cytosolic fractions were used for Western Blot analysis. Expression of Bax, Bcl-2, and Bad was not affected by mPEPCK suppression. (c) Altered phosphorylation level of main signaling molecules after mPEPCK suppression in SNU-C4 cells. Phosphorylation levels of AKT and 4EBP1 were significantly decreased in SNU-C4 cells after mPEPCK siRNA transfection.