Figure 4

Effect of mPEPCK overexpression on 5-FU response in SNU-C4 cells. Overexpression of mPEPCK in (a) SNU-C4 and (c) LoVo transfected with pEGFPc1-mPEPCK. Upregulated mPEPCK was monitored over 24-, 48-, 72-, and 96-h time periods by Western Blot, while the pEGFPc1-transfected control showed no changes in PEPCK expression levels. No effect of overexpression of mPEPCK was observed in (b) SNU-C4 or (d) LoVo on the susceptibility to 5-FU, irradiation, or combination treatment comprising 5-FU and irradiation. Overexpression of mPEPCK slightly increased the susceptibility to 5-FU in both SNU-C4 and LoVo cells, but the difference was not statistically significant.