| Control cells | WT-Ras cells |
---|
– | TNFα | – | TNFα |
---|
Exp #1
| 1.00 | 2.64 | 1.62 | 3.39 |
Exp #2
| 1.00 | 2.94 | 3.29 | 4.64 |
Exp #3
| 1.00 | 2.78 | 1.98 | 4.60 |
- MCF-7 cells were transfected with WT-Ras vector or with control vector, and were not-stimulated or stimulated by TNFα (50 ng/ml). Stimulation of the transcriptional activity of NF-κB was determined in cells transfected to express the luciferase gene under the control of 3 conserved repeats of NF-κB binding sites, using dual luciferase assay. Control vector-transfected non-stimulated cells were given the value of 1. The table presents the results obtained in 3 independent experiments, whose average results are shown in Figure 4B. Please see “Methods” for additional details on the experimental procedures performed in this part of the study.