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Table 1 The cooperativity of WT-Ras and TNFα stimulates the transcriptional activity of NF-κB

From: The inflammatory cytokine TNFα cooperates with Ras in elevating metastasis and turns WT-Ras to a tumor-promoting entity in MCF-7 cells

 

Control cells

WT-Ras cells

TNFα

TNFα

Exp #1

1.00

2.64

1.62

3.39

Exp #2

1.00

2.94

3.29

4.64

Exp #3

1.00

2.78

1.98

4.60

  1. MCF-7 cells were transfected with WT-Ras vector or with control vector, and were not-stimulated or stimulated by TNFα (50 ng/ml). Stimulation of the transcriptional activity of NF-κB was determined in cells transfected to express the luciferase gene under the control of 3 conserved repeats of NF-κB binding sites, using dual luciferase assay. Control vector-transfected non-stimulated cells were given the value of 1. The table presents the results obtained in 3 independent experiments, whose average results are shown in Figure 4B. Please see “Methods” for additional details on the experimental procedures performed in this part of the study.