Figure 2

TNFα and WT-Ras cooperate in inducing CXCL8 up-regulation. (A) Induction of CXCL8 at the mRNA level, determined by qRT-PCR in MCF-7 cells transfected to over-express ErbB2 or control vector, and stimulated by EGF (30 ng/ml). (B) Induction of CXCL8 at the protein level, determined by ELISA in CM of MCF-7 cells transfected to express Ras^G12V, WT-Ras or the appropriate control vector. (C, D) CXCL8 induction in MCF-7 cells transfected to express WT-Ras and stimulated by TNFα (50 ng/ml), determined at the protein level in cell CM by ELISA (C) and at the mRNA level by qRT-PCR (D). *p < 0.05, **p < 0.01, ***p < 0.001 compared to control transfectants (A, B), or to non-stimulated cells (C, D). ¹, Not in all assays this value was significant. NS = Not significant. In all panels, a representative experiment of n≥3 is presented. Please see “Methods” for additional details on times of CM collection, and of mRNA analyses.