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Figure 4 | BMC Cancer

Figure 4

From: Tumor-stroma metabolic relationship based on lactate shuttle can sustain prostate cancer progression

Figure 4

Stimulation of glycolysis in human fibroblasts. Diploid human fibroblasts WI-38 were cultured in presence of 30% PC3-conditioned medium (CM), or two concentrations of Cobalt(II)Chloride (CoCl2, 100 and 200 μM). A) After 24 h, cell media were collected and the rate of secreted L-lactate was calculated normalizing for protein content and time of culture. B) The same experimental conditions were repeated in presence of 50 μM HIF-1α inhibitor (iHIF-1α) and total cell lysates from fibroblasts were analyzed by western blot for the expression of MCT4 and HIF-1α. Values from densitometric analysis are shown at the bottom of each protein band and were calculated as described in the Methods section. C) Representative images taken by phase contrast microscopy of WI-38 cells cultured for 48 h in presence of 30% PC3 conditioned medium and 50 μM HIF-1α inhibitor. In insets the mean percentage ± SD of viable WI-38 cells from three different experiments is shown.

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