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Figure 3 | BMC Cancer

Figure 3

From: Tumor-stroma metabolic relationship based on lactate shuttle can sustain prostate cancer progression

Figure 3

Effects of exogenous L-lactate on prostate cells. A) WPE1 and LNCaP cells were treated with 5 and 10 mM L-lactate for 24 h and total cell lysates were subjected to western blot analysis for MCT1, AMP activated protein kinase- alpha (AMPK), total and active form (Thr172), cytochrome c oxidase subunit 1 (COX1). Beta-actin detection was utilized as loading control. Values from densitometric analysis are shown on top of each protein band and were calculated as described in the Methods section. B) In the same experimental condition MCT1 and COX1 expression was detected also by quantitative RT-PCR (right histogram). C) Proliferation assay in control-silenced (CTR-sil) prostate cells, MCT1-silenced (MCT1-sil) prostate cells cultured for 48 h in different glucose concentrations (1, 0.6 g/L) and in presence or not of 5 mM L-lactate (lac). Cells cultured in 0.6 g/L glucose without L-lactate were chosen as reference (100%). Data represent mean values ± SD from at least three independent experiments. *P < 0.01 according to Student’s t test.

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