Figure 1

L-lactate export and glycolytic metabolism in PCa cell lines, LNCaP and PC3, and in transformed epithelial cell lines, RWPE-1 and WPE1-NB26 (WPE1). A) L-lactate export rate was calculated considering measurements at 24, 48 and 72 h in standard culture conditions and normalizing for total protein content (μg). B) Western blot analysis of MCT4 in whole cell lysates from PCa cell lines, LNCaP and PC3, and from transformed epithelial cell lines, RWPE-1 and WPE1. Beta-actin detection was utilized as loading control. C) Immunofluorescence detection of MCT4 in PC3 and LNCaP cells. In the image showing MCT4 expression in LNCaP cells, nuclei have been visualized by DAPI staining. Arrows indicate the positive staining for MCT4 mainly localized in the center of cell aggregate. D) Percentages of viable cells treated with 10 μM oligomycin for different times in comparison with untreated cells. E) Anti-proliferative effect of glycolysis inhibitors 2-deoxy-D-glucose (2-DG, 10 mM) and 3-bromo-pyruvate (3-BP, 50 μM). Percentages of viable cells respect to untreated cells (100%) are reported. Data represent mean values ± SD from at least three independent experiments.