The role of CXC-chemokine receptor CXCR2 and suppressor of cytokine signaling-3 (SOCS-3) in renal cell carcinoma

BMC Cancer

Table 2 Characteristics of primary antibodies used in immunohistochemical analysis

Protein	No of cases	Clone	Company	Catalog no.	Raised in	Positive controls	Antigen retrieval method	Dilution and incubation time for immunohistochemistry
IL-8	118	Polyclonal	Invitrogen Corporation, Camarillo, CA	AHC 0881	Rabbit	Normal tonsillar tissue	pH 6 (low)	1:50, 18 h 4°C
IL-6	118	Polyclonal	Santa Cruz Biotechnology, Santa Cruz, CA	SC 1265	Goat	Normal tonsillar tissue	pH 6 (low)	1:50, 18 h 4°C
SOCS-3	118	Polyclonal	Santa Cruz Biotechnology, Santa Cruz, CA	SC 9023	Rabbit	Cholangiocarcinoma	pH 6 (low)	1:100, 18 h 4°C
CXCR2	118	Monoclonal	R&D Systems, Abingdon, England	MAB 331	Mouse	Normal tonsillar tissue	pH 9 (high)	1:100, 18 h 4°C
VEGF	117	Monoclonal	Pharmingen BD Company, San Diego, CA	clone G153-694	Mouse	Glioblastoma	pH 6 (low)	1:40, 18 h 4°C
p-STAT3 [specific at site Tyr 705]	117	Monoclonal	Cell Signaling Technology Inc., Boston, MA, USA	D3A7 XP	Rabbit	Human breast cancer	pH 6 (low)	1:100, 18 h 4°C
CD31	111	Monoclonal	DAKO	clone JC70A	Mouse	Kaposi sarcoma	pH 9 (high)	1:20, 18 h 4°C
P53	30	Monoclonal	DAKO	IR616	Mouse	Serous ovarian carcinoma	pH 6(low)	Pre-diluted, 1 h 37°C
HIF-1a	30	Monoclonal	Neomarkers Inc. Fremont CA, USA	MS 1164P	Mouse	Glioblastoma	pH 6(low)	Pre-diluted, 1 h 37°C
p65/RelA	30	Polyclonal	Zymed laboratories Inc., California, USA	18-7308	Rabbit	breast carcinoma	pH 6(low)	1:300, overnight 37°C

ISSN: 1471-2407