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Table 2 Characteristics of primary antibodies used in immunohistochemical analysis

From: The role of CXC-chemokine receptor CXCR2 and suppressor of cytokine signaling-3 (SOCS-3) in renal cell carcinoma

Protein No of cases Clone Company Catalog no. Raised in Positive controls Antigen retrieval method Dilution and incubation time for immunohistochemistry
IL-8 118 Polyclonal Invitrogen Corporation, Camarillo, CA AHC 0881 Rabbit Normal tonsillar tissue pH 6 (low) 1:50, 18 h 4°C
IL-6 118 Polyclonal Santa Cruz Biotechnology, Santa Cruz, CA SC 1265 Goat Normal tonsillar tissue pH 6 (low) 1:50, 18 h 4°C
SOCS-3 118 Polyclonal Santa Cruz Biotechnology, Santa Cruz, CA SC 9023 Rabbit Cholangiocarcinoma pH 6 (low) 1:100, 18 h 4°C
CXCR2 118 Monoclonal R&D Systems, Abingdon, England MAB 331 Mouse Normal tonsillar tissue pH 9 (high) 1:100, 18 h 4°C
VEGF 117 Monoclonal Pharmingen BD Company, San Diego, CA clone G153-694 Mouse Glioblastoma pH 6 (low) 1:40, 18 h 4°C
p-STAT3 [specific at site Tyr 705] 117 Monoclonal Cell Signaling Technology Inc., Boston, MA, USA D3A7 XP Rabbit Human breast cancer pH 6 (low) 1:100, 18 h 4°C
CD31 111 Monoclonal DAKO clone JC70A Mouse Kaposi sarcoma pH 9 (high) 1:20, 18 h 4°C
P53 30 Monoclonal DAKO IR616 Mouse Serous ovarian carcinoma pH 6(low) Pre-diluted, 1 h 37°C
HIF-1a 30 Monoclonal Neomarkers Inc. Fremont CA, USA MS 1164P Mouse Glioblastoma pH 6(low) Pre-diluted, 1 h 37°C
p65/RelA 30 Polyclonal Zymed laboratories Inc., California, USA 18-7308 Rabbit breast carcinoma pH 6(low) 1:300, overnight 37°C