Figure 7

Ki-67 protein expression in MCF10A cells with various localization of maspin. Cells were transfected with plasmids EGFP, maspin-EGFP and maspin-NLS-EGFP and fixed after 24 to 120 hours; a, b - the majority of cells transfected with all three constructs express Ki-67 protein at 48 h and 120 h after transfection; c - maspin localization in MCF10A cells after transfection; MCF10A cell line expresses endogenous maspin which is mostly localized in cytoplasm; d - statistical analysis of Ki-67 expression in MCF10A cells transfected with three plasmids; there are no significant differences at 24 h to 120 h after transfection between cells expressing nucleus-localized maspin and those expressing both cytoplasm-localized maspin and EGFP.