Figure 6

Analysis of Ki-67 protein expression in MDA-MB-231 and SKBR-3 cells with various localization of maspin. Cells were transiently transfected with plasmids EGFP, maspin-EGFP and maspin-NLS-EGFP and fixed 24 to 120 hours later; a, b, d, e - the majority of cells transfected with all three constructs express Ki-67 protein at 48 h and 96 h after transfection; c, f - the majority of cells expressing nucleus-localized maspin do not express Ki-67 at 120 h after transfection; simultaneously there is no such observation in control cells and cells expressing EGFP and msp-EGFP localized in cytoplasm; arrows show cells expressing nuclear maspin that lost Ki-67 protein.