Figure 5

Statistical analysis of cell proliferation and Ki-67 expression in transfected MCF-7 cells. Control cells and cells transfected with three plasmids - EGFP, maspin-EGFP and maspin-NLS-EGFP - were subjected to statistical analyses of proliferation and Ki-67 protein expression; a, b - cell proliferation assay at 24 h to 9 days after transfection; only after 96 h and 144 h were there significant differences between cells expressing cytoplasmic and nuclear maspin, but generally cells in both transfections died rapidly; however, there were new colonies in MCF-7 msp-EGFP cells, in contrast to msp-NLS-EGFP cells; c, d - percent of transfected cells expressing Ki-67 protein; there are significant differences at 72 h after transfection between cells expressing nucleus-localized maspin and those expressing both cytoplasm-localized maspin and EGFP; these differences increase at 96 h and 120 h after transfection.