Phospho-aspirin-2 inhibits the growth of TNBC cells. A:
Left: Chemical structure of phospho-aspirin-2 (PA-2, MDC-22). Right: 24 h-IC50 values of PA-2 and aspirin in TNBC cell lines. B: MDA-MB-231 and BT-20 cells were treated with PA-2 for 24 h and the percentage of proliferating cells was determined by BrdU incorporation. C: MDA-MB-231 and BT-20 cells treated with PA-2 for 24 h were stained with Annexin V/PI, and the percentage of apoptotic cells was determined by flow cytometry. D: PA-2 blocks the G1/S cell cycle phase transition after 24 h treatment in MDA-MB-231 cells, determined by flow cytometry following PI staining.