TaqMan qRT-PCR confirmation of IR-A/IR-B mRNA ratio in NSCLC primary tumors using cDNA array. The IR-A/IR-B mRNA ratio was assessed by determining ΔCt differentials of IR-A and IR-B in lung cancer and normal lung samples. The ΔCt differential (IR-B ΔCt – IR-A ΔCt) values were calculated for each specimen utilizing the within-specimen reference gene panel (average Ct) and normalized to the average of the normal lung samples. The error bars represent the mean -ΔCt ± 95% CI within a particular gene target and sample-type combination. Dotted line indicates average levels of IR-A/IR-B ratios in normal lung tissues.