TaqMan qRT-PCR confirmation of relative mRNA Expression Levels for IR-A and IR-B in NSCLC primary tumors run on cDNA arrays. TaqMan gene expression assays determined the relative mRNA expression levels of IR-A and IR-B. For each sample, the expression levels of IR-A and IR-B were normalized to the average expression levels of 3 housekeeping genes (ACTB, GAPDH and UBC). The relative expression levels of IR-A and IR-B are represented by -ΔCt, where -ΔCt = − [(Ct of a gene of interest) - (Average Ct of 3 housekeeping genes)]. The error bars represent the mean -ΔCt ± 95% CI within a particular gene target and sample-type combination. (A): data from cDNA array, (B) data from primary tumor samples. Asterisks indicate statistically significant differences (* = P < 0.05; ** = P < 0.01; *** = P < 0.001).