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Figure 2 | BMC Cancer

Figure 2

From: Increased IR-A/IR-B ratio in non-small cell lung cancers associates with lower epithelial-mesenchymal transition signature and longer survival in squamous cell lung carcinoma

Figure 2

TaqMan qRT-PCR confirmation of relative mRNA Expression Levels for IR-A and IR-B in NSCLC primary tumors run on cDNA arrays. TaqMan gene expression assays determined the relative mRNA expression levels of IR-A and IR-B. For each sample, the expression levels of IR-A and IR-B were normalized to the average expression levels of 3 housekeeping genes (ACTB, GAPDH and UBC). The relative expression levels of IR-A and IR-B are represented by -ΔCt, where -ΔCt = − [(Ct of a gene of interest) - (Average Ct of 3 housekeeping genes)]. The error bars represent the mean -ΔCt ± 95% CI within a particular gene target and sample-type combination. (A): data from cDNA array, (B) data from primary tumor samples. Asterisks indicate statistically significant differences (* = P < 0.05; ** = P < 0.01; *** = P < 0.001).

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BMC Cancer

ISSN: 1471-2407

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