Figure 2

Classification of 14 HNC lines using immunohistochemistry (IHC) marker parameters. (A) Representative example of a combined IHC marker staining for PIMO (green), CA9 (red) and vessel (blue) staining. (B + C) Expression of an endogenous hypoxia marker (CA9) and an exogenous hypoxia marker (PIMO) in the different tumor lines (mean ± SD). (D) Clustered heatmap of the IHC parameters with overall good clustering of the different tumor lines. Tumor lines are indicated by their respective numbers. (E) Graph displaying an estimate of the decrease in Random Forest classification accuracy when omitting the respective parameter. (F) Random Forest classification accuracy as a function of the (randomly combined) number of IHC parameters. * = significantly different from previous number of parameters (t-test).