Skip to main content

Table 1 Summary of the properties of the evaluated methods

From: Comparison of high resolution melting analysis, pyrosequencing, next generation sequencing and immunohistochemistry to conventional Sanger sequencing for the detection of p.V600E and non-p.V600E BRAFmutations

  HRM Pyro Cobas IHC NGS Sanger
CE mark No No Yes No No No
Limit of detection [%] 6.6 5 7 5 2 6.6
Detection of rare mutations Yes Yes- downstream of codon 600 No- 4 different mutations No- p.V600E Yes Yes
Specificity [%] 100 90 98.3 98 100 100
Turnaround time 1 day 2 days 1 day 1 day 3 - 5 days 2 - 3 days
Costs Low High Medium Low Very high Medium
  1. HRM: high resolution melting analysis; Pyro: pyrosequencing with the therascreen® BRAF Pyro Kit; cobas: cobas® BRAF V600 test; IHC: immunohistochemistry; NGS: next generation sequencing on the MiSeq platform; Sanger: Sanger sequencing.