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Table 1 Summary of the properties of the evaluated methods

From: Comparison of high resolution melting analysis, pyrosequencing, next generation sequencing and immunohistochemistry to conventional Sanger sequencing for the detection of p.V600E and non-p.V600E BRAFmutations

 

HRM

Pyro

Cobas

IHC

NGS

Sanger

CE mark

No

No

Yes

No

No

No

Limit of detection [%]

6.6

5

7

5

2

6.6

Detection of rare mutations

Yes

Yes- downstream of codon 600

No- 4 different mutations

No- p.V600E

Yes

Yes

Specificity [%]

100

90

98.3

98

100

100

Turnaround time

1 day

2 days

1 day

1 day

3 - 5 days

2 - 3 days

Costs

Low

High

Medium

Low

Very high

Medium

  1. HRM: high resolution melting analysis; Pyro: pyrosequencing with the therascreen® BRAF Pyro Kit; cobas: cobas® BRAF V600 test; IHC: immunohistochemistry; NGS: next generation sequencing on the MiSeq platform; Sanger: Sanger sequencing.