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Figure 2 | BMC Cancer

Figure 2

From: Comparison of high resolution melting analysis, pyrosequencing, next generation sequencing and immunohistochemistry to conventional Sanger sequencing for the detection of p.V600E and non-p.V600E BRAFmutations

Figure 2

BRAF p.V600E mutation analysis of formalin-fixed paraffin-embedded tumor samples using Sanger sequencing. Electropherograms showing that Sanger sequencing is not only able to detect mutations with high mutant allele frequency (D - F) but also mutations with rather low mutant allele frequency (B and C) compared to a wildtype sample (A). Even 6.6% of BRAF p.V600E allele could be detected. A: adenine, C: cytosine, G: guanine, T: thymine, T: threonin, V: valine, K: lysine.

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