Figure 2

Representative images of FISH and H&E staining from tumour and histologically benign areas of the same case. A: an H&E image of a tumour section (from Case 01, tumour block) with the area of tumour circled; B: MYC amplification (green signals indicated by the red arrow) and DAPI nuclear staining (blue) in a representative field of view of the tumour section; C: PTEN deletion indicated by green arrows and DAPI nuclear staining (blue) in a representative field of view of the tumour section (gold PTEN signals are visible only in stroma cells); D: an H&E image of the histologically benign section (from Case 01, histologically benign block) with the area that has abnormal FISH signals circled; E: MYC amplification (green signals indicated by the red arrow) and DAPI nuclear staining (blue) in a representative field of view of the histologically benign section; F: PTEN deletion indicated by green arrows and DAPI nuclear staining (blue) in a representative field of view of the histologically benign section (gold PTEN signals are visible only in stroma cells, indicated by white arrows).