Figure 6
From: Estrogen receptor α-coupled Bmi1 regulation pathway in breast cancer and its clinical implications
Interaction of ERα and Bmi1 promoter. MCF-7, MDA-MB-231 and 231/ERα treated or untreated with 10−8 M E2 for 12 h were used for Chromatin immunoprecipitation (ChIP) analysis of the human Bmi1 promoter as described in Methods. ERα: anti-ERα antibody; IgG: rabbit IgG. Bmi1 promoter: region (−327 bp to −172 bp), and control: region (−2647 bp to −2523 bp). PCR products were resolved on a 2% agarose gel.