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Figure 5 | BMC Cancer

Figure 5

From: Estrogen receptor α-coupled Bmi1 regulation pathway in breast cancer and its clinical implications

Figure 5

Depletion of ERα decreased the level of Bmi1 and its E2 response. (A) MCF-7 cells were transiently transfected with three sets of siRNAs specific for ERα (siRNA1, siRNA2, siRNA3) or with a control smart pool siRNA (NS), and 72 h after transfection cells were analyzed by Western blot (left panel). Quantitative analyses of ERα protein expression by the three siRNAs are presented (right panel). (B) MCF-7 cells were transiently transfected with siRNA3, and Bmi1, ERα and p16INK4a were analyzed by Western blot (left panel) and real time RT-PCR (right panel). β-actin was used as loading control. Quantitative analyses of ERα, Bmi1 and p16INK4a are presented (middle panel). Right panel: quantitative analyses on mRNA of ERα, Bmi1 and p16INK4a. All data were obtained from three independent experiments and are shown by bars as means ± SD (**,## or P < 0.01, ***P < 0.001 when ERα, Bmi1 and p16INK4a were compared with the NS group, respectively).

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