Regulation of CD248 in cancer cells. (A, B) Wehi-231 (A) and A20 (B) mouse lymphoma cells were incubated with different concentrations of TGFβ for 24 or 48 hrs and lysates were assessed by Western immunoblot. CD248 levels were minimally affected in spite of phosphorylation of Smad2. Results are representative of 3 independent experiments. (C) Normal fibroblasts (NF) and cancer associated fibroblasts (CAF) from murine mammary tissue were exposed to TGFβ for 24 or 48 hrs and CD248 mRNA levels were quantified and normalized to levels from untreated NF. CD248 mRNA levels in NF were significantly suppressed by TGFβ, whereas there was no effect on CD248 in CAF. *p < 0.05, n = 3.