Stability of CD248 mRNA is unaffected by TGFβ. MEF were treated with TGFβ 3 ng/ml alone, α-amanatin 20 μg/ml alone, or with a combination of TGFβ and α-amanitin as described in Methods. CD248 mRNA levels, relative to the mRNA levels of the housekeeping gene GAPDH, were quantified at different time intervals by qRT-PCR. Results were normalized from 3 independent experiments, each done in triplicate. The half-life of CD248 mRNA is approximately 75 minutes, which is unaltered by TGFβ.