Figure 5

Vimentin is a direct target of miR-378. (A) The wild-type (WT) and mutated (MUT) 3′UTR of vimentin, with the seed region and base substitutions (bold). (B) The expression levels of vimentin mRNA and protein were detected by qRT-PCR and western blot assays. (C) Ectopic miR-378 expression inhibits wild-type but not mutant vimentin 3′UTR reporter activity. *P < 0.05, **P < 0.01.