Figure 1

Primary data for surgical samples from a 68-year-old woman (study patient). a. Down-regulation of the COL1A1 gene was seen in the tumor tissue compared with the adjacent noncancerous liver tissue (146 bp). Reverse transcriptase polymerase chain reaction (RT-PCR) for β-actin was performed to normalize the quantity of cDNA. b Copy number analysis of chromosome 17. There was no deletion or amplification at the COL1A1 gene locus (17q21.33). c Immunohistochemical staining of COL1A1 protein showed that tumor tissue components showed almost no staining compared with adjacent noncancerous tissue components (200×).